To gain information about the cellular tolerance of the developed polyelectrolyte complex a new, innovative multilayered-stratified human cornea cell model was used for determination of the cellular toxicity in vitro. A heat induced stress test was carried out to ensure the stability of the polyelectrolyte complex.
The complex was characterized in detail by attenuated total reflection infrared spectroscopy, X-ray powder diffraction, and differential scanning calorimetry. The Amphotericin B polyelectrolyte complex (AmpB-Eu L) performed best and was very effective against the fungal strain Issatchenkia orientalis in vitro. These include bile salt addition, encapsulation in poly(lactic-co-glycolic acid) (PLGA) nanoparticles and formation of a polymeric Amphotericin B polyelectrolyte complex. Different formulation strategies were applied to increase the drug load of the sparingly water-soluble Amphotericin B in electrospun Gellan Gum/Pullulan fibers. The purpose of our research was the development of Amphotericin B-loaded in situ gelling nanofibers for the treatment of keratomycosis. 5Department of Ophthalmology, Martin Luther University Halle-Wittenberg, Halle, Germany.4Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark.3Leibniz Institute of Surface Engineering (IOM), Leipzig, Germany.2Interdisciplinary Center of Materials Science, Martin-Luther University Halle-Wittenberg, Halle, Germany.1Pharmaceutical Technology and Biopharmacy, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, Halle, Germany.Benedikt Göttel 1, Henrike Lucas 1, Frank Syrowatka 2, Wolfgang Knolle 3, Judith Kuntsche 4, Joana Heinzelmann 5, Arne Viestenz 5 and Karsten Mäder 1 *
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